The Preparation of retrieved cells to make them suitable for transplantation denotes Cell manipulation.
Recent advances in microfluidic technology for manipulation and analysis of biological cells are Advances in microfluidic technology for the cell analysis in the last decades that were reviewed. Microfluidic platforms exhibited superior spatiotemporal resolution for cell manipulation and monitoring of cellular responses. The commercialization of microfluidic technology has gained traction in biomedical research and diagnostic applications.
Figure 1: Showing the Microfluidic Palette
Digital microfluidics is an important method for cell manipulation. Digital microfluidics (DMF) operating discrete droplets for cell culture, cell sorting, and single-cell analysis. Suspension cell, microorganism, two-dimensional (2D) adherent cell and three-dimensional (3D) cell culture happens on the DMF devices. Cell sorting and concentrating happens within DMF droplets with integrated electrical, optical and magnetic forces. Single-cell culture, stimulation, immunocytochemistry, DNA analysis, mRNA extraction and transcriptome analysis happens with the DMF. Unlimited Genetic Switches for Cell-Type-Specific Manipulation is a new technology to gain independent genetic access to discrete cell types. The multiplexing potential of CRISPR exploited to create infinite genetic switches. A gene-trap system is generally used to discover the lineage-specific genes. The multi-gene intersectional genetics is a vertebrate and invertebrate model.
SponGee is a Genetic Tool for Subcellular and Cell-Specific cGMP Manipulation. The cGMPsometime becomes critical to some of the variety of cellular processes. However, the available tools to interfere with endogenous cGMP lack cellular and subcellular specificity. The SponGee is a genetically encoded chelator of this cyclic. SponGee is a genetically encoded cGMP scavenger. SponGee inhibits cGMP-dependent downstream pathways. SponGee enables cell-specific manipulation of cGMP-dependent processes in vivo. Subcellular targeting confers cell compartment specificity to SponGee.
Figure 2: Showing Cell Manipulation process
Cell Manipulation Technologies:
Activities of cells are dependent on extracellular niches. The change of the cells is changing quite dynamically. Dynamic substrates are there whose chemical or physical properties can be controlled by an external stimulus. These properties are useful to resolve how cells respond to such dynamic environmental changes. They are useful for cell manipulations, such as heterotypic cell coculturing, cell sheet harvesting, and cell migration induction. We have developed for the first time a dynamic substrate, which is based on the photocleavage reaction of the 2-nitrobenzyl group. One of the biggest advantages of the photochemical approach is its high spatiotemporal resolution. However, there is a lot of room for innovation to improve the switching efficiency and to expand the time window of its usage.
Pinpoint Cell Penetrator for Targeted Microinjection 